cyclin-dependent kinase 2 (cdk2) primary antibody  (Thermo Fisher)

Journal: Biomolecules & Therapeutics

Article Title: Antitumor Effects of Fucoidan on Human Colon Cancer Cells via Activation of Akt Signaling

doi: 10.4062/biomolther.2014.136

Figure Lengend Snippet: Fucoidan-induced activation of the Akt pathway and Akt inhibitor-mediated reversal of p21WAF1 and CDK expression. (A) HT29 colon cancer cells were treated with fucoidan (100 μg/mL) for various times (0–8 h). The activation of Akt was analyzed by western blot analysis using a phospho-Akt antibody. (B) HT29 colon cancer cells were pretreated for 4 h with an Akt inhibitor (10 −6 M) and then incubated with or without fucoidan for 6 h. The activation of Akt was analyzed by western blot analysis using a phospho-Akt antibody. (C and D) HT29 colon cancer cells were pretreated for 4 h with an Akt inhibitor (10 −6 M) and then incubated with or without fucoidan for 24 h. Western blot analysis was performed with antibodies specific for p21WAF1, CDK2, and CDK4. The lower panel depicts the mean ± SEM of three independent experiments for each condition, as determined from densitometry relative to β-actin. * p <0.05 and ** p <0.01 vs. control, # p <0.05 and ## p <0.01 vs. fucoidan only group, $ p <0.05 and $ p <0.01 vs. Akt inhibitor only group.

Article Snippet: The membranes were blocked with 5% skim milk and incubated with primary antibodies against vascular endothelial growth factor (VEGF), phospho-AKT, cyclin-dependent kinase 2 (CDK2), CDK4, Cyclin D, Cyclin E, p21, and β-actin (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Activation Assay, Expressing, Western Blot, Incubation, Control